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Our team is working on the development of innovative therapies for the treatment of genetic pathologies that affect the retina (retinopathies). We base our work on the potential of human pluripotent stem cells.

Degenerative diseases of the retina are a major cause of vision loss throughout the world. Today, they often remain incurable (only a few drugs exist: LuxturnaTM, gene therapy for Leber’s Amaurosis (RPE65); SyfovreTM, anti-complication for atrophic AMD and anti-VEGF for the wet form of AMD) and lead more or less rapidly to blindness in patients. In France, approximately 40,000 people are affected by hereditary diseases and more than 1.5 million by age-related macular degeneration (AMD). Progressive and very disabling, retinal diseases are characterized by a progressive degeneration of the cells lining the retina, particularly the photoreceptors. The most numerically important inherited degenerative retinal diseases are the pigmentary retinopathies. These diseases are caused by more than 70 genes and can cause visual impairment at any age.

The team’s project is articulated around 2 main axes:
• The production of functional retinal progenitors from human pluripotent cells (hPSC) for use in cell therapy. Because of their pluripotency and their capacity to differentiate infinitely, hPSCs represent a source of retinal progenitors that can be used in cell therapy. The team is developing protocols for guided differentiation of pluripotent cells towards different cell populations of the retina (retinal pigment epithelium cells, (RPE) and photoreceptors). Part of these protocols has been adapted on a culture automaton allowing large-scale culture of these cells (Régent et al., 2019). Moreover, in collaboration with Dr Hamouda (Université Paris-Saclay) and Pr Letourneur (Inserm U698), we have developed cell engineering products that combine matrices/scaffolds and cells recapitulating the cellular organization of the retina. A clinical cell therapy program that started in 2019 is currently underway (NCT03963154).

• The study of genetic retinal pathologies using hPSC lines carrying mutations, with the aim of identifying biological markers associated with the mutation and then initiating the screening of potentially therapeutic molecules (Lustremant et al., 2013; Régent et al.). Our studies focus on Alström syndrome.

Team members

Christelle MONVILLE

Team Leader, Pr. & VP Research

Professor, Université Evry-Paris Saclay.
Vice-President of Research (university) since 2023 January.


Researcher (CECS)

Researcher in the retinopathy team since its creation, Walter is specialized in the use of retinal epithelial cells derived from pluripotentstem cells for cell therapy approach.


Research Technician (CECS)

A member of the Retinopathies team at I-Stem from the outset, Alexandra is involved in the STREAM cell therapy project, as well as the team’s other research projects. She is working on the immunological characterization of retinal pigment epithelium cells.


Researcher (CECS)

Karim arrived in 2012 as an INSERM post-doc after a thesis done at the Institut Curie, and has been a research fellow in the team since 2017. He obtained my HDR in 2022.


PhD student

He arrived in February 2020 as a study engineer and is continuing his research as a thesis student at the University of Evry. His research project aims at developing a cellular model of Alström syndrome, a ciliopathy characterized by retinitis pigmentosa.

Camille GEIGER

Engineer (CECS)

Camille joined I-Stem in April 2021 as a design engineer following a Master’s degree in Biotechnology. Her project involves bioengineering retinal tissue for use as a cell therapy product.



Joining the Retinopathies team in September 2023 as a post-doctoral fellow, Hélène is seeking to establish a 3D cell co-culture model of the blood-retinal barrier with a view to cell therapy.


Apprentice Technician (CECS)

Natacha PERRIN

Assistant researcher (CECS)

Natacha has been a research engineer in the Retinopathies team since January 2024. She works in collaboration with the Centre de Nanosciences et de Nanotechnologies (C2N) on a project to reconstruct the retina using tissue bioengineering.


Dr. Olivier Goureau & Dr Emeline Nandrot
IDV, Inserm UMR S968, Paris, France, (ANR GPiPS)
Dr. Didier Letourneur
Inserm U698, Paris, France

Use of Biodegradable Matrices for the culture and transplantation of cells derived from hES.

Dr Catalin Fetita
Telecom SudParis
Dr Fréderic Hamouda
C2N, Université Paris-Saclay
Dr Brigitte Malgrange
GIGA, Université de Liège, Belgique
Dr Vincent Gasque
Pr. Stéphane Blot
Laboratoire de Neuroscience, ENVA, Maisons-Alfort, France

ANR Cardiostem

Dr. Bijan Ghaleh & Pr Alain Berdeaux
Inserm U955, Créteil, France

ANR Cardiostem


Advances in the engineering of the outer blood-retina barrier: From in-vitro modelling to cellular therapy.

01 January 2024

Dujardin Chloé,

Habeler Walter,

Monville Christelle,

Letourneur Didier,

Simon-Yarza Teresa,

Bioactive materials

Semi-automated optimized method to isolate CRISPR/Cas9 edited human pluripotent stem cell clones.

27 April 2023

Frank Elie,

Cailleret Michel,

Nelep Constantin,

Fragner Pascal,

Polentes Jérome,

Herardot Elise,

El Kassar Lina,

Giraud-Triboult Karine,

Monville Christelle,

Ben M'Barek Karim,

Stem cell research & therapy

Photoreceptor Cell Replacement Using Pluripotent Stem Cells: Current Knowledge and Remaining Questions.

01 February 2023

Monville Christelle,

Goureau Olivier,

Ben M'Barek Karim,

Cold Spring Harbor perspectives in medicine